考马斯亮蓝法测定(实验报告)(2)

2.2不同缓冲体系对可溶性蛋白提取效果的影响

2.2.1不同缓冲溶液对可溶性蛋白提取效果的影响

表三水及不同pHTris-HCI对可溶性蛋白提取效果的影响

溶液及P H直水PH=7.2PH=7.4PH=7.6PH=7.8PH=8.0PH=8.2PH=8.4PH=8.6PH=8.8PH=9.0吸光度A0.1290.1510.180.1630.1660.1600.1720.1860.2010.2180.258

蛋白含量卩g19.1923.2923.1125.5326.0924.9727.2029.8132.6135.7843.23

蛋白含量%15.3518.6318.4920.4220.8719.9821.7623.8526.0928.6234.58