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目的 研究脂类干扰剂非律平和制霉菌素对人羊膜上皮细胞鞘脂类代谢的影响是否相同。 方法?┯τ没?质辅助激光解吸附飞行时间质谱分析方法分析不同剂量非律平和制霉菌素对人FL细胞株鞘脂类代谢的影响。 结果 非律平和制霉菌素均可影响FL细胞鞘脂类代谢,但是非律平(0.2 和10 μm
中国药理学与毒理学杂志 2007年10月;21(5)·421·
Effectsoffilipinandnystatinonsphingolipidsmetabolismin
humanamnionepithelialcells
12211?
LIUGuang?Yi,MAXiao?Qiong,SHENJing,DONGZheng?Wei,YANGJun
(1.DepartmentofToxicology,SchoolofPublicHealth,2.DepartmentofPathologyandPathophysiology,
SchoolofMedicine,ZhejiangUniversity,Hangzhou 310058,China)
Abstract:AIM Tostudywhethermacrolides
polyeneantibioticsfilipinandnystatinhavedif?ferenteffectsonsphingolipidsmetabolismin
(FL)cells.METH?humanamnionepithelial
AfterFLcellsweretreatedwithtwoODS
0.2and10μmol·concentrationsoffilipin(
-1L)andnystatin(0.054and0.108μmol·-1L),respectively,sphingolipidswereextrac?
,laserde?tedandsubjectedtomatrix?assisted
sorption?ionizing?timeofflightmassspectrome?
ESULTS Bothfilipinandnys?tryanalysis.R
tatinaffectedsphingolipidsmetabolism.How?
-1
,filipin(0.2and10μmol·L)mainlyeveraffectedceramide,andinducedthegenerationofmanynewceramidespecies.Ontheother
-1
hand,nystatin(0.054and0.108μmol·L)affectedbothceramideandsphingomyelin.Inadditiontotheinductionofnewceramidespe?
,nystatinalsoincreasedtheconcentrationcies
ofseveralsphingomyelinspecies.Nonetheless,thehigherconcentrationofnystain(0.108
-1
mol·L)inducedthegenerationofmorece?μramidespeciesthanthelowerconcentrationof
-1
0.054μmol·L).CONCLUSIONnystatin(
Bothfilipinandnystatinaffectsphingolipids
,howevertheymaytargetdifferentmetabolism
stepsinthesphingolipidsmetabolismpathway.Keywords:filipin;nystatin;lipidomics;ce?
;sphingomyelinsramides
oundationitem:TheprojectsupportedbyNationalNat? F
(30300277);NationalNaturaluralScienceFoundationofChina
ScienceFoundationofChina(30471956);andNationalNaturalScienceFoundationofChina(30600220)
?
Correspondingauthor.
(本文编辑 齐春会)

