非律平和制霉菌素对人羊膜上皮细胞鞘脂类代谢的影响(5)

睡觉也偷笑 分享 2021-09-28 下载文档

[19] ChuCL,Buczek?ThomasJA,NugentMA.Heparansul?

phateproteoglycansmodulatefibroblastgrowthfactor?2bindingthroughalipidraft?mediatedmechanism[J].BiochemJ,2004,379(Pt2):331-341.

[20] OgretmenB,HannunYA.Biologicallyactivesphingo?

lipidsincancerpathogenesisandtreatment[J].NatRevCancer,2004,4(8):604-616.

[21] ReynoldsCP,MaurerBJ,KolesnickRN.Ceramide

synthesisandmetabolismasatargetforcancertherapy[J].CancerLett,2004,206(2):169-180.

[[[[

目的 研究脂类干扰剂非律平和制霉菌素对人羊膜上皮细胞鞘脂类代谢的影响是否相同。 方法?┯τ没?质辅助激光解吸附飞行时间质谱分析方法分析不同剂量非律平和制霉菌素对人FL细胞株鞘脂类代谢的影响。 结果 非律平和制霉菌素均可影响FL细胞鞘脂类代谢,但是非律平(0.2 和10 μm

中国药理学与毒理学杂志  2007年10月;21(5)·421·

Effectsoffilipinandnystatinonsphingolipidsmetabolismin

humanamnionepithelialcells

12211?

LIUGuang?Yi,MAXiao?Qiong,SHENJing,DONGZheng?Wei,YANGJun

(1.DepartmentofToxicology,SchoolofPublicHealth,2.DepartmentofPathologyandPathophysiology,

SchoolofMedicine,ZhejiangUniversity,Hangzhou 310058,China)

Abstract:AIM Tostudywhethermacrolides

polyeneantibioticsfilipinandnystatinhavedif?ferenteffectsonsphingolipidsmetabolismin

(FL)cells.METH?humanamnionepithelial

 AfterFLcellsweretreatedwithtwoODS

0.2and10μmol·concentrationsoffilipin(

-1L)andnystatin(0.054and0.108μmol·-1L),respectively,sphingolipidswereextrac?

,laserde?tedandsubjectedtomatrix?assisted

sorption?ionizing?timeofflightmassspectrome?

ESULTS Bothfilipinandnys?tryanalysis.R

tatinaffectedsphingolipidsmetabolism.How?

-1

,filipin(0.2and10μmol·L)mainlyeveraffectedceramide,andinducedthegenerationofmanynewceramidespecies.Ontheother

-1

hand,nystatin(0.054and0.108μmol·L)affectedbothceramideandsphingomyelin.Inadditiontotheinductionofnewceramidespe?

,nystatinalsoincreasedtheconcentrationcies

ofseveralsphingomyelinspecies.Nonetheless,thehigherconcentrationofnystain(0.108

-1

mol·L)inducedthegenerationofmorece?μramidespeciesthanthelowerconcentrationof

-1

0.054μmol·L).CONCLUSIONnystatin(

Bothfilipinandnystatinaffectsphingolipids

,howevertheymaytargetdifferentmetabolism

stepsinthesphingolipidsmetabolismpathway.Keywords:filipin;nystatin;lipidomics;ce?

;sphingomyelinsramides

 

oundationitem:TheprojectsupportedbyNationalNat?  F

(30300277);NationalNaturaluralScienceFoundationofChina

ScienceFoundationofChina(30471956);andNationalNaturalScienceFoundationofChina(30600220)

?

  Correspondingauthor.

(本文编辑 齐春会)


非律平和制霉菌素对人羊膜上皮细胞鞘脂类代谢的影响(5).doc 将本文的Word文档下载到电脑

下一篇:美国的行政机关与宪法实施的监督_王宝明

相关推荐
相关阅读
本类排行
× 游客快捷下载通道(下载后可以自由复制和排版)

下载本文档需要支付 7 元

支付方式:

开通VIP包月会员 特价:29元/月

注:下载文档有可能“只有目录或者内容不全”等情况,请下载之前注意辨别,如果您已付费且无法下载或内容有问题,请联系我们协助你处理。
微信:xxxxxx QQ:xxxxxx