中二个软ON OF IL 8IN RATS WITH GASTRIC ULC ER
Ling Jiang hong,Li Jiabang,Shen Ding zhu,et al.(Institute of Integrated T raditional Chinese and West Medicine,Xiangya H ospital,Centr al South Univer sity,Changsha410008China)
Abstract Objective:To inv estig ate the effect o f Jianw eiyuyang granules(JWYY)on activation of nuclear B(NF B)and the ex pr ession o f IL 8in r ats w ith g astric ulcer,and to discuss the probable mechanism of its g astric m ucosa protective effect.Methods:56SD rats w ere random ly div ided into4g roups:norm al gro up,sham operation g roup,model g roup,and JWYY g roup.T he g astric ulcer w as induced in the r ats by acetic acid acco rding Okabe's m ethod w ith some mo dification.Im munohisto chem istry(IH)and In Situ H y bridization H istochemistr y(ISH)w er e used to detect the expressio n of NF Bm RNA and its pro tein as w ell as IL 8protein in the sto mach.Result:The8th day after the model w as made,NF BmRNA and its protein w ere significantly translocated fro m the cytoplasm into the nucleus,how ever,NF BmRNA and its protein remained in the cy to plasm of gastr ic epithelium in normal and sham oper ation gr pared to the nor m al g roup(0.41 0.17,0.29 0.11)、sham o peratio n g roup(0.54 0.15,0.38 0.15),their cell density of positiv e reactio n in nucleus increased(5.92 0.61,9.13 1.21,all P<0.01),so did IL 8pro tein(58.95 7.34vs9.55 1.58,11.00 2.21,both P<0.01).NF BmRNA and its pr otein significantly shifted out fro m nucleus to cytoplasm on the16th days.Cell density of po sitive reaction w as observed decreasing in NF BmRNA and its pro tein(2.42 0.51,4.18 0.94,both P<0.01)as w ell as IL 8(15.72 2.67,P<0.
