落尽梨花年产1000吨苏云金芽孢杆菌的发酵车间工艺设计
of Bacillus thuringiensis[J]. Biotechnology Letters,2001, 23: 1201-1204.
[14] 余健秀, 汤慕谨, 徐建敏, 等. 苏云金杆菌小试生产发酵影响因子的研究[J]. 中国生物防治, 2002, 18 (1): 17-20.
YU Jianxiu, TANG Mujin, XU Jianmin, et al. The Factors Affectingthe Fermentation of Bacillus thuringiensis[J]. Chinese Journal of BiologyControl, 2002, 18 (1): 17-20.
[15] SAKHAROVA Z V, RABOTNOVA I L, KHOVRYCHEV M P. Growthand spore formation in Bacillus thuringiensis var. israelensis and developmentof media for production of protein Crystal
endo-toxin[J].Biotechnology Letters, 1988, 10(7): 451-456.
[16] YASEMIN I, BüLENT I, GüLAY ?. Regulation of Crystal protein biosynthesis by Bacillus thuringiensis: Ⅱ. Effects of carbon and nitrogen sources[J]. Research in Microbiology,
2002, 153: 605-609.
[17] SALAMA H S, FODA M S, Dulmage H T. Novel fermentation media for production of δ-endotoxins from Bacillus thuringiensis[J]. J Invert Pathol, 1983, 41: 8-19.
[18] YASEMIN I, BüLENT I, GüLAY ?. Regulation of Crystal protein biosynthesis by Bacillus thuringiensis: Ⅰ. Effect of mineral elements and pH[J]. Research in Microbiology, 2002,
153: 599-604.
[19] SACHDEVA V, TYAGI R D, VALéRO J R. Factors affecting the production of Bacillus thuringiensis biopesticides[J]. Recent Research and Development of Microbiology, 1999 (3):
363-375.
[20] MUMMIGATTI S G, RAGHUNATHAN N. Influence of media composition on the production of δ-endotoxin by Bacillus thuringiensis var. thuringiensis[J]. Journal of Invertebrate Pathology, 1990, 55: 147-151.
[21] LACHHAB K, TYAGI R D, VALéRO J R. Production of Bacillus thuringiensis biopesticides using wastewater sludge as a raw material: effect of inoculum and sludge solids concentration[J]. Process Biochemistry, 2001, 37: 197-208.
[22] WAKISAKA W, MASAKI E, NISHIMOTO Y. Formation of Crystalline δ-endotoxin or poly-β-hydroxybutyric acid granules by asporogenous mutants of Bacillus thuringiensis. Applied and Environmental Microbiology, 1982: 43.
[23] SIKDAR D P, MAJUMDAR M K, MAJUMDAR S K. Effect of minerals on the production of the delta endotoxin by Bacillus thuringiensis subsp. israelensis[J]. Biotechnology Letters, 1991, 13(7): 511-514.
[24] ABROSIMOVA L I, AVA P V, BREVA G M, et al. Influence of mineral salts on the level of exotoxin production and productivity of a culture of Bacillus thuringiensis[J]. Microbiology, 1986, 55(3): 337-341.
[25] ZOUARI N, JAOUA S. The effect of complex carbon and nitrogen, salt, Tween-80 and acetate on delta-endotoxin production by a Bacillus thuringiensis var. kurstaki[J]. Journal of Industrial Microbiology and Biotechnology, 1999, 23: 497-502.
[26] AVIGNONE-ROSSA C A, YANTORNO O M, ARCAS J A, et al. Organic and inorganic nitrogen source ratio effects on Bacillus thuringiensis var. israelensis delta-endotoxin production[J]. World Journal of Microbiology and Biotechnology, 1990, 6: 27-31.
31
年产1000吨苏云金芽孢杆菌的发酵车间工艺设计
[27] ABDEL-HAMEED A, CARLBERG G, ELTAYEB O M. Studies on Bacillus thuringiensis H-14 strains isolated in Egypt-Ⅳ. Characterization of fermentation conditions for delta-endotoxin production. World Journal of Microbiology and Biotechnology[J]. 1991, 7(2): 231-236. [28] MIRTA M F, OSVALDO Ｍ Y, HORACIO A, et al. Effect of the media composition on the growth parameters and biological properties of Bacillus thuringiensis var. israelensis delta-endotoxin[J]. World Journal of Microbiology and Biotechnology, 1990, 6: 32-38. [29] TIRADO M M L, TYAGI R D, VALéRO J R, et al. Production of biopesticides using wastewater sludge as a raw material-effect of process parameters[J]. Water Science and Technology, 2003, 48(8):239-246.
[30] KANG B C, LEE S Y, CHANG H N. Enhanced Spore Production Of Bacillus thuringiensis By Fed-Batch Culture[J]. Biotechnology Letters,1992, 14 (8): 721-726.
[31] 朱玮, 赵兵, 王晓东, 等. 生物农药苏云金芽孢杆菌的研究进展[J].过程工程学报, 2004, 4(3): 282-288.
ZHU Wei, ZHAO Bing, WANG Xiaodong, et al. Progress of the Study on Bacillus thuringiensis as Biopesticides[J]. The Chinese Journal of Process Engineering, 2004, 4(3): 282-288. [32] HUANG T K, WANG P M, WU W T. Cultivation of Bacillus thuringiensis in an Airlift Reactor with Wire Mesh Draft Tubes[J]. Biochemical Engineering Journal, 2001, 7: 35-39. [33] DHOUHA G, NABIL Z, HASSEN T. Improvement of Bacillus thuringiensis delta-endotoxin production by overcome of carbon catabolite repression through adequate control of aeration[J]. Enzyme and Microbial Technology, 2007, 40(4): 614-622.
[34] ACRAS J, YANTORNO O, ERTOLA R. Effect of High Concentration of Nutrients on Bacillus thuringiensis Culture[J]. Biotechnology Letters, 1987, 9(2): 105-110.
[35] ZHOU Jingwen, CHANG Yafei, XU Zhenghong. Production of thuringiensin by fed-batch culture of Bacillus thuringiensis subsp. Darmstadiensis 032 with an improved pH-control glucose feeding strategy[J]. Process Biochemistry, 2007, 42 (1): 52-56.
[36] 杨淑兰，张怡，梁淑兰，等. 生物杀虫剂苏云金杆菌的固态发酵[J].化工冶金, 1993, 14(2): 162-167.
YANG Shulan, ZHANG Yi, LIANG Shulan, et al. Solid fermentation of Bacillus thuringiensis based biopesticide[J]. Journal of Chemical Metallurgy, 1993, 14(2): 162-167.
[37] CAPALBO D M F, MORAES I O, PELIZER L H. Solid-State fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize. Electronic Journal of
Biotechnology, 2001, 4(2): 112-115.
[38] VIMALA D P S, RAVINDER T, JAIDEV C. Cost-effective production of Bacillus thuringiensis by solid-state fermentation[J] Journal of Invertebrate Pathology, 2005,88(2): 163-168. [39] 陈洪章, 李佐虎. 固态发酵新技术及其反应器的研制[J]. 化工进展, 2002, 21(1): 37-40. CHEN Hongzhang, LI Zuohu. Study on Solid-state Fermentation and Fermenter[J]. Chemical Industry and Engineering Progress, 2002,21(1): 37-40.
[40] TYAGI R D, SIKATI F V, BARNABE S, et al. Simultaneous production of biopesticide and alkaline proteases by Bacillus thuringiensis using sewage sludge as a raw material[J]. Water Science and Technology,2002, 46(10): 247-254.
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年产1000吨苏云金芽孢杆菌的发酵车间工艺设计
附录
附录一 生产原料
物质名称 啤酒糟
外观 浅黄色至深黄色
其他性质
啤酒糟主要由麦芽的皮壳、叶芽、不溶性蛋白质、半纤维素、脂肪、灰分及少量未分解的淀粉和未洗出的可溶性浸
出物组成。
米粉
浅黄色至深黄色粉末，无虫蛀、无霉变 米粉，是指以大米为原料，经浸泡、蒸
煮、压条等工序制成的条状、
丝状米制品
豆粕
豆粕一般呈不规则碎片状，颜色为浅黄豆粕的主要成分为：蛋白质40%～48%，色至浅褐色，味道具有烤大豆香味
赖氨酸2.5%～3.0%，色氨酸0.6%～
0.7%，蛋氨酸0.5%～0.7%
KH2P03
深紫色或古铜色结晶。无臭。味甜而涩 溶于14.2份冷水、3.5份沸水。相对密
度2.703
附录二 设备一览表
主要设备一览表
设备名称 发酵罐 种子罐 储罐 过滤器 离心机 干燥机 离心泵 搅拌槽
参数 4000nmx8000nm 40nmx80nm 1800nmx3400nm 390nm×390nm v=13m3
WG=3500r/min 2300nm×3220nm×2000nm
R=2900r/min WG=1500r/min 台数 3 3 4 1 1 1 10台以上
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年产1000吨苏云金芽孢杆菌的发酵车间工艺设计
工艺流程图
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附录三
年产1000吨苏云金芽孢杆菌的发酵车间工艺设计
附录四 设备布局图
35
年产1000吨苏云金芽孢杆菌的发酵车间工艺设计
致 谢
通过努力这次设计顺利完成了，并最终定稿，看着自己的劳动结晶，内心充满了感激。在此，我向我的指导老师张秀云老师、陈光友老师表示最衷心的感谢！同时，我也感谢我的所有任课老师，还有感谢“中心”在这次设计完成过程中也给予的大量支持和帮助，在此谨表示诚挚的谢意！在完成设计的过程中，我们专业的同学之间互相讨论，互相帮助，我真心感谢全专业同学的支持与帮助！
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