High expression of Cullin1 indicates poor prognosis for NSCLC patients(4)

M.Xuetal./Pathology–ResearchandPractice210(2014)397–401

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Fig.1.Expressionpro lesofCullin1inNSCLCandnon-tumorousadjacenttissues.

Immunohistochemicalevaluation

Theintensityanddistributionpatternsofthestainingreactionwereevaluatedby3blinded,independentpathologists.Forassess-mentofCullin1andKi-67, vehigh-power eldsineachspecimenwereselectedrandomly,cytoplasmandnuclearstainingwereexamined.Morethan1000cellswerecountedtodeterminethemeanpercentage,whichrepresentedthepercentageofimmunos-tainedcellsrelativetothetotalnumberofcells.Inmorethanonehalfofthesamples,stainingwasrepeatedthreetimestoavoidtechnicalerrors,andaconsensuswasachieved.ToevaluatetheCullin1proteinimmunoreaction,stainingintensewasclassi edtobe0(negatively,poorlyormoderatelystaining),1(stronglystain-ing).WhenevaluatingtheKi-67proteinimmunoreaction,stainingwasscoredinasemi-quantitativefashion.Acut-offvalueof50%ormorepositivelystainednucleiwasusedtode neKi-67staining:lowexpressiongroup(<50%)andhighexpressiongroup(≥50%).

mainlyinthenuclei(Fig.2).Tumorigenesiswasassociatedwithmultiplefactors,andCullin1overexpressionmightbeanimpor-tantfactorinNSCLC.Thus,wehypothesizedthatoverexpressionofCullin1mightcontributetotheproliferationofNSCLC.