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应用RSAP、SRAP和SSR分析苎麻种质亲缘关系
邹自征;陈建华;栾明宝;郭劲霞;王超;王晓飞;许英;孙志民
【期刊名称】《作物学报》 【年(卷),期】2012(038)005
【摘要】The research on molecular biology in ramie [Boehmeria nivea (L.) Gaud.] is not as advanced as that in major crops due to the lagged development of molecular markers. We compared the effectiveness of restriction site amplified polymorphism (RSAP), sequence-related amplified polymorphism (SRAP), and simple sequence repeat (SSR) markers in revealing genetic relationship of 16 entries of ramie germplasm. The SRAP marker detected the most polymorphic loci, followed by the RSAP marker, and SSR marker had the lowest polymorphism. The dendrogram based on SRAP or RSAP data showed high
consistency
with
that
based
on
combined
data
of
SRAP+RSAP+SSR, and their genetic distances were significantly correlated. In contrast, the SSR data constructed a dendrogram with different clusters of the 16 entries. The similarity of clusters between phenotypic traits and molecular markers was sequenced as SRAP+RSAP+SSR > RSAP > SRAP > SSR. The SPAR marker had the highest efficiency to disclose the genetic relationship among ramie germplasm, which was slightly higher than that of the RSAP marker, whereas the SSR showed the lowest efficiency. Thus, RSAP marker was