loading索要质粒的邮件模板
Dear Professor Bruss V:
I am working on immunology. And one of my projects focuses on the treatment of HBV.We want to construct
a
vector
pHBV1.5
which
your
used
in
one
of
your
paper(Proc.Natl.Acad.Sci.USA,vol88:1059-1063).Construct this vector seems to be a problem hard to get over for us.One of my graduates has been working on it for a couple of months, but no progress. We are happy to find that your laboratory was the major source of the pHBV1.5. Therefore, I am writing to explore the possibility of getting pHBV1.5 from your laboratory. If you were kindly offering us the vector, it would be very helpful for us. Of course, the plasmid will never be used for commercial purpose.We will acknowledge the plasmid resource and cite your papers in all our publications based on the plasmid. Sure, We would like to sign any contract for the plasmid transfer.
One of my graduates is waiting for the vector to set up his experiment, so your early response will be greatly appreciated.
结果给我寄过来了,他是用滤纸寄的,先用500ng质粒溶解在1ulTE里,然后滴在滤纸上用塑料袋封闭,用的时候同样使用50ulTE将质粒溶解在EP管中,短暂离心后上清即可用于细菌转化.他是用空寄的,所以不需要通过海关,一般半个月就到了.